Effect of Electrical Field Treatment Variation against Lethal Level of MCF-7 (Human Breast Cancer) Cell Line in vitro and Cell Capacitance Value Measurement
Qolby Sabrina, MS Thesis, Dept. of Physics, University of Indonesia, 2014
Breast cancer therapy using electric field called Electro-Capacitive Cancer Therapy (ECCT) has been developed previously. Result of in vitro experiment showed that electric field with an 18 Volt rectangular output signal can inhibit cancer cell growth and cause injury that leads to cell death. In this research the in vitro experiment was performed on MCF-7 (human breast cancer) cell line with signal and voltage variation of output electric field. Then the number of cells was counted with a hemocytometer and the cell capacitance was measured before and after treatment with the electric field with observation time 24, 48 and 72 hours. The experiment results show that the standard ECCT with 18 Volt rectangular output signal and non-standard ECCT with 31.2 Volt rectangular output signal can inhibit cell growth. Cell morphology results show injury that indicates cell deaths, while standard ECCT with 18 Volt sinusoidal output signal was able to induce cells to grow more after electric field treatment marked by the high growth coefficient. Measuremen of cell capacitance values showed a correlation between the number of cells with the value of the measured capacitance. The high number of cells indicates the high malignancy of cancer cells with high cell electrical activity thus capacitance.
MCF-7 cell line , ECCT, cancer cell growth, capacitance cell measurement