Cell Death Mechanism Result of Weak Energy AC Electric Field with Intermediate Frequency (Experimental Research In Vitro, ECCT on Cell Cultures of HeLa, Oral Cancer and Bone Marrow Mesenchyme)
Sahudi, PhD Dissertation, Dept. of Medicine, Airlangga University, 2015
Background. This study was conducted to answer the controversy about ECCT that uses low-voltage electric field with medium frequency.
Research Purpose. Proving the existence of the increasing percentage cell death by ECCT exposure, and uncover the molecular mechanism of pathology.
Research Method. This study was an experimental laboratory in vitro using Completely Randomized Block Design, and aimed to determine the effect of exposure to low-voltage electric field with intermediate frequency of ECCT. Three kinds of cancer cell cultures used in this study were HeLa cells, Oral Cancer cells, and Bone Marrow Mesenchyme cells. All three were divided into two groups with each 8 replication, which is the treatment group that would be exposed with ECCT for 24 hours, and the control group. Living cells and dead cells were calculated using Tryphan Blue staining, and the protein expression of TubulinA, CyclinB, p53, and Ki-67 were examined.
Result. From this study, the cell group that has been given the ECCT exposure showed significantly more number and percentage of cell death than the control group, and this occured in both the cancer cells and non-cancerous cells. On the viability of cells, ECCT had significantly decreased the number of living cells, whereas in non-cancerous cell cultures, which is bone marrow mesenchymal cells, ECCT had affected the number of its living cell, but not statistically significant. ECCT exposure for 24 hours had significantly increase the expression of Tubulin A, Cyclin B1, p53, and Ki-67.
Conclusion. Low-voltage AC Electric field with intermediate frequency from ECCT can kill cancer cells through mechanism of mitotic catastrophe.
Keyword: ECCT, in vitro, cell culture, Tubulin A, Cyclin B1, p53, Ki-67, mitotic catastrophe