Anti-proliferative effect of electric fields on breast cancer cells in vitro and in vivo

    Firman Alamsyah1, Izzatun Niswah Ajrina2, Fitriya Nur Annisa Dewi3,4, Diah Iskandriati3,4, Silvia Arin Prabandari4 and Warsito P. Taruno1

    International Conference on Advanced Pharmaceutical Sciences and the 6th Symposium of ISCC (Indonesian Society for Cancer Chemoprevention), 14 – 16 Sept 2015, Pontianak – Indonesia

    1Center for Medical Physics and Cancer Research Ctech Labs Edwar Teknologi,

    2Dept. of Biology Faculty of Science and Technology Airlangga University,

    3Bimana Indomedical,

    4Primate Research Center Bogor Agricultural University

    Corresponding Author Email: firman.alamsyah@c-techlabs.com

    ABSTRACT:

    Our research focused on the anti-proliferative effect of low intensity (18 Vpp) and intermediate frequency (100 KHz) electrostatic wave between two capacitive electrodes on breast cancer cells in vitro and in vivo. In vitro study has been conducted using MCF-7 cell lines at 24, 48, and 72 hours of treatment. In vitro study revealed 28-39% growth inhibition efficacy of MCF-7 cells. For in vivo, we used female mice (Mus musculus) strain C3H as animal model. The mice were injected with either MCF-7 cells, mammary tumor cells from C3H donor, or NaCl 0.9% (placebo) subcutaneously into the axilla area. Furthermore, the placebo and tumor animals were exposed to external electrostatic wave individually in their cages. After 2 weeks of exposure, placebo mice were physically normal, whereas the tumor undergone significant shrinkage of more than 67% in size. Histopathological analysis of the mammary glands indicated inflammation in the tumor area with macrophages infiltrate through the blood vessel. No abnormality was found in the skin layer and mammary glands of the breast tissue of placebo mice. Here, we present new knowledge of electro-capacitive cancer therapy (ECCT) as a novel treatment modality.

     

    Keywords:

    ECCT, tumor, in vitro, in vivo